The Natural resources Essay Example for Free

The Natural resources Essay The Natural resources company in the oil production exploration field had huge number of projects to work on including 50% platform projects, 30% medium 20% small scale projects, 80000 hours are required to cover all the projects. 41 employees are currently overseeing the project management department where, an average of 5 employees contributes in the major project 8 for minor medium each. At present, many projects in particular the minors, have crossed their deadlines budget. The reason; too much labour being utilized in the major projects, creating trouble for the employees as well as the company’s budget. To retain a balanced portfolio, project resources will have to be split equally across the three types of Projects i. e. 33. 3% by reducing the platform projects from 5 to 3 and increasing Small Scale Projects from current 18 to about 22. The main issues strategies of the company can be observed by conducting an overall analysis, which can help in accomplishing a thriving portfolio. All these steps can be taken successfully, only if the support of management is present the new users made aware of the implementation of the plan through regular meetings in order to obtain their contribution via feedback. Due to the oil prices on a high rate, the company is gaining in the financial perspective therefore; creating prospects for new projects hence expands the portfolio. Ensuring the quality of the product at a competitive price can be achieved through effective project management. Reducing the operating costs delivering on time will help to increase profits by securing clients. This prospect is being incorporated by involving local firms in the company’s projects. Increasing the number of employees will let us take up more projects saving time consequently gaining revenue. The proposed stratagem should fit the culture of the company and the community or they would face customers’ rejection.

Construction of Recombinant DNA in E Coli

Construction of Recombinant DNA in E Coli In 1973 Stanley Cohen and Herbert Boyer pioneered the use of recombinant DNA technology for cloning and expression of genes in foreign organisms. They cloned DNA from the Salmonella typhimurium streptomycin resistance plasmid RSF1010 into the Escherichia coli plasmid pSC101 and observed tolerance to streptomycin among the transformants (Cohen et al., 1973). The first reported production of a human recombinant protein took place a few years later when the then newly started biotech company Genentech announced that they had managed to express the gene encoding human somatostatin in E. coli (Itakura et al., 1977). The value of the resulting bioactive substance was similar to that of somatostatin extracted from the brains of 500.000 sheep. In 1982 Genentech followed up this success with the product humulin, a recombinant insulin produced in E.coli and the first recombinant biotech drug to be accepted for market by the Food and Drug Administration. Today the production of recombinant prot eins has become a huge global industry with an annual market volume exceeding $50 billion (Schmidt, 2004). At the start of the recombinant protein expression era the bacteria Escherichia coli and Bacillus spp. dominated as hosts for recombinant expression, but the realization that a protein may require a specific host physiology and biochemistry for optimal production stimulated a search for new hosts, both prokaryotic and eukaryotic. Parallel to this quest, recombinant DNA technology advanced tremendously thereby opening up possibilities for the use of novel organisms. As a consequence, many different expression systems for use in many different hosts are now available, including systems for use in yeasts (Gellissen et al., 2005), filamentous fungi (Nevalainen et al., 2005), insect and animal cell cultures (Wurm, 2004; Kost et al., 2005), gram-positive bacteria like Bacillus (Westers et al., 2004) and Streptomyces (Binnie et al., 1997), and gram-negative bacteria like Escherichia c oli Bacterial expression systems are the preferred choice for production of many prokaryotic and eukaryotic proteins. The reasons for this lie in the cost-effectiveness of bacteria, their well-characterized genetics, and the availability of many different bacterial expression systems. Among the hosts available for recombinant expression, Escherichia coli is in an exceptional position. This stems from the many decades of intense researchon its genetics as well as the broad scope of biotechnological tools available for genetic engineering of this organism. As a host for recombinant expression, E.coli is especially valued because of its rapid growth rate, capacity for continuous fermentation, low media costs and achievable high expression levels (Yin et al., 2007). One consequence of this popularity is that about 80% of all proteins used to solve three-dimensional structures submitted to the protein data bank (PDB) in 2003 were prepared in E.coli (SÃ ¸rensen and Mortensen, 2005) and during 2003 and 2006, nine out of 31 approved therapeutic proteins were produced in E.coli (Walsh, 2006), among them important growth factors, insulins and interferons (Schmidt, 2004). Green Fluorescent Protein (GFP) was isolated from the jellyfish Aequorea aequorea in 1962 (Shimomura et al., 1962) where it was found as a companion protein to aequorin, the well-known chemiluminescent protein of the same species. It was noticed that living A. aequorea tissue had an emission spectrum peaking at 508nm and looking green but pure aequorin peaked in the blue range, at 470nm (Tsien, 1998). This then led Shimomuras group to discover GFP and suggest radiation-less energy transfer as the mechanism for exciting the protein. Its structure has been determined to consist of an 11 stranded ÃŽÂ ²-barrel containing the chromophore made up of a single ÃŽÂ ± helix as shown in Figure1. Its use as a tool in molecular biology was not realised until 1992 when Prasher reported the cloning and sequence of GFP (Prasher et al., 1992). Since 1994 GFP has been used as a reporter protein (Chalfie et al., 1994) flagging its own presence and therefore also proteins under the same control, by emitting green light (ÃŽÂ »em = 508 nm) upon excitation with near ultraviolet light (around 395 nm) or blue light (around 470 nm) (Ito et al, 1999). Since then many mutations have been developed looking to improve the emission or to focus it to a single wavelength (Heim et al., 1995) or to change the color of the emitted light itself. Recombinant DNA molecules usually contain a DNA fragment inserted into a bacterial vector. Polymerase chain reaction (PCR), a specific gene or DNA region of interest is isolated and amplified by DNA polymerase extracted from a heat-tolerant bacteria. PCR finds the DNA region of interest (called the target DNA) by the complementary binding of specific short primers to the ends of that sequence. The long chromosome-size DNA molecules of genomic DNA must be cut into fragments of a much smaller size before they can be inserted into a vector. Most cutting is done with the use of bacterial restriction enzymes. These enzymes cut at specific DNA sequences, called restriction sites, and this property is one of the key features that make restriction enzymes suitable for DNA manipulation. These enzymes are examples of endonucleases that cleave a phosphodiester bond (Anthony, 2012). The key property of some restriction enzymes is that they make sticky ends. The restriction enzyme EcoRI (from E.coli) recognizes the following sequence of six nucleotide pairs in the DNA of any organism: 5-GAATTC-3 3-CTTAAG-5 The enzyme EcoRI makes cuts only between the G and the A nucleotides on each strand of the palindrome (Figure.2). The recombinant DNA molecules are transferred into bacterial cells, and, generally, only one recombinant molecule is taken up by each cell. The recombinant molecule is amplified along with the vector during the division of the bacterial cell. This process results in a clone of identical cells, each containing the recombinant DNA molecule, and so this technique of amplification is called DNA cloning. The next stage is to find the rare clone containing the DNA of interest. Bacterial plasmids (vectors) are small circular DNA molecules that replicate their DNA independent of the bacterial chromosome. The plasmids routinely used as vectors carry a gene for drug resistance and a gene to distinguish plasmids with and without DNA inserts. These drug-resistance genes provide a convenient way to select for bacterial cells transformed by plasmids: those cells still alive after exposure to the drug must carry the plasmid vectors. However, not all the plasmids in these transformed cells will contain DNA inserts. For this reason, it is desirable to be able to identify bacterial colonies with plasmids containing DNA inserts. Such a feature is part of the pUC18 (or pUC19) plasmid vector shown in Figure 2; DNA inserts disrupt a gene (lacZ) in the plasmid that encodes an enzyme (-galactosidase) necessary to cleave a compound added to the agar (X-gal) so that it produces a blue pigment. Thus, the colonies that contain the plasmids with the DNA insert will be white rath er than blue (they cannot cleave X-gal because they do not produce -galactosidase). The following experiment outlines the construction of recombinant protein production in E.coli strain BL21 by using a bacterial plasmid vector pUC18/19 expressing Green Fluorescent Protein (GFP) to act as a recombinant protein product with the benefits of being easy to visualise and measure. Materials and Methods Materials: The experiment was carried out using the following materials and Equipments: 2Â µl EcoRI/HindIII cut and cleaned PUC19 vector, 5Â µl EcoRI/HindIII cut and cleaned GFP insert, 2Â µl 10xT4 ligase buffer, 2Â µl T4 ligase(0.5 U ml-1) , and 9Â µl sterile water (H2O) ]to make up to 20Â µl volume[ . 100Â µl of competent BL21 E.coli cells on ice, 42Â °C water bath, Ice bucket with ice, selective media plates (1.5% Luria broth (LB) Agar, 40Â µg mL-1 X-gal, .1 mM IPTG, 50Â µg mL-1 ampicillin), sterile tubes, shaking incubator, Spectrophotometer or similar device to measure optical density of the bacterial cultures, flasks, Microcentrifuge. Methods: It can be divided into three stages: Ligation Reaction stage: in this stage 2Â µl EcoRI/HindIII cut and cleaned PUC19 vector, 5Â µl EcoRI/HindIII cut and cleaned GFP insert, 2Â µl 10xT4 ligase buffer, 2Â µl T4 ligase (0.5 U ml-1) , and 9Â µl sterile water (H2O) are mixed and kept at room temperature for at least 30 minutes. Transformation of ligation into cloning host stage: this stage conducted by deforesting 100Â µl of competent BL21 E.coli cells on ice (with caution do not allow to warm to room temperature), then adding 10Â µl of the ligation reaction from the first stage to BL21 E.coli cells. They are then incubated for up to 30 minutes on ice. Next step, is done by taking out the transformation mixture out of the ice and heated in water bath at 42 Â °C for almost 75 seconds, then followed by return immediately into ice for a minimum of 2 mins. Then the cells were plated out on selective media plates (1.5% Luria broth (LB) Agar, 40Â µg mL-1 X-gal, .1 mM IPTG, 50Â µg mL-1 ampicillin). Lastly, the transformation mixture is incubated at 37 Â °C for 12-18 hours afterdriedd. Picking of colonies for the protein expression stage: 2x5ml LB +50Â µg ml-1 ampicillin in 30ml sterile tubes were prepared, then 1xBlue individual colony and 1x white individual colony selected and inoculated in separate tubes. Then the tubes were incubated with shaking incubator throughout the night at 37Â °C , speed: 220rpm. Subculture and Growth of Recombinant E.coli for Protein expression: At the beginning, 2x60ml sterile Luria-Bertani (LB), in 250ml conical flask were warmed , (1 per inoculums ) at 37 Â °C, Then aseptically the ampicillin was added to a last concentration of 50Â µg ml-1 ampicillin. Next 1 ml of media was removed and was put in a cuvette to act as blank (one blank is enough for both ouh), followed by addition of 600Â µl overnight to calture of each individual colony to separate flask (1:100 inoculum), the flasks were put back to the shaking incubator and incubated at 37Â °C, speed: 200rpm , after that blank spectrophotometer was placed against media at 600nm , after 45 minutes the samples were removed aseptically from flasks, then from every flask 1x 1mL was removed and added to a fresh clean cuvette (take to next step 8) and 1x1ml was added to clean Eppendrof (take to step 9) . The OD600nm of culture in cuvette was Measured and the result of growth curve was recorded (once the cul ture has reached an OD 600nm of 0.5, IPTG was added to final concentration 1Mm stock solution. Then samples were spun down in the Eeppendrof tube at max speed in Microcentrifuge for 5 minutes , ensure centrifuge is balanced before spinning , the supernatant was removed and pellet ,then the pellet was suspended in 200Â µl Cell lysis buffer (10mMl Tris PH8.0, 300Mm Nacl , 10mg ml-1 Lysozyme). Resuspended cells were frozen at -20 c to the next day. Lastly, sampling was continued until OD600nm is no longer rising for two successive samples or until 16:30 pm. Results and discussion Although it is supposed to harvest between 30-300 colonies per plate (210- 2100 colonies for all groups), just three blue colonies were observed in plates between all groups, which mean that protein of interest (GFP protein ) was not expressed (inefficient) in BL21 E.coli cells due to some factors influenced the expression level or to some technical problems during the experiment which will be discussed. The most popular strain, BL21 and its derivatives, which are good producing protein, are descended from E.coli B and thus is deficient in the Lon protease. Additionally, the BL21 background lacks the OmpT outer membrane protease. For expression work, BL21 cells should be taken from stock cultures that performed from fresh transforms. This step is crucial to insure that the clone does not change and that each expression run gives optimal performance. Transformation frequency is affected by the purity of the DNA, how the cells are handled, and how the transformation was actually performed. In the impurities in the DNA usually spin columns can be used to purify DNA from PCR reactions, ligations, endonuclease digestions, or other treatments. In addition, the most common mistake when transforming E.coli is to put a lot of ligation mixture in the transformation. Other factors that effect transformation with BL21 are the handling and the storage of the competent cells. Competent cells need to be reserved at -70Â °C to keep them at the peak .It is worthy of noting that 5-10-fold of efficiency usually lost if tube put back in the box and place in the freezer. Moreover, Cells must be thawed on ice, and the transformation should be started immediately after the cells are thawed. Incubating on ice is necessary for chemically competent cells. If you heat shock right away, the efficiencies will be down 10-fold. If incubate for only 15 minutes, it will be down 3-fold. In addition, time of heat shock (75 second) could be not enough , thus, affect the efficiency enough to transformation of E.coli. Moreover, water bath temperature may be not equilibrated (less than 42Â °C or a higher which decrease in transformation efficiency ( Smith, et al, 1992). Also, the concentration of DNA has significant effect on the transformation efficiency , usually less amount of DNA is used. If using more, the result is fewer colonies because the impurities in the DNA will inhibit some of the cells from being transformed. There are main factors to consider during induction conditions: Vector, Host Strain, and Growth Conditions. These three factors have the biggest impact on the expression of the protein of interest. First on the list of considerations is the vector that is used to express GFP protein. The first thing should be considered after cloning, the protein of interest is still in frame. It is recommended that before any experiment is carried out the first thing is should be done is cloned plasmid (or a few different clones) sequenced. This will show if the sequence you inserted into the expression vector is still correct and is still in frame. This is especially important if the construct contains any PCR fragments. If there are any point mutations or the sequence gets out of frame by even a few bases it can have dramatic effects on the protein that expressed. Another thing to check before expressing is if the GFP protein sequence contains long stretches of rare codons. This can cause the prot ein that is expressed to be truncated or non-functional. A few rare cordons spread around the protein are OK in most cases, but if there are a number of rare codons in a row, then it can have a big effect. The third sequence related step to optimize the protein production is to make sure there is not a high GC concentration at the 5 end of GFP protein. This could potentially cause problems with the mRNAs stability, and could prevent it from being translated correctly, which would also lead to truncated or non-functional proteins. If your sequence is GC heavy at this end, you can try to make a few silent mutations to break up long stretches to try and help stability. After the plasmid is sequence verified, the next factor is the bacterial host that is used. There are almost as may hosts as there are expression vectors, with certain hosts excelling in producing different types of proteins. For example if you have a toxic protein, or a protein that could potentially cause genomic rearrangement, you will want a vector that gives you very tight control over the induction of your protein. There can be leaky expression (i.e. expression of your protein without the addition of your inducer) that can potentially have adverse effects on the cells growth or even prevent your cells from over-expressing your protein in the first place. If youre utilizing the T7 polymerase system, then look for a host containing the pLysS plasmid, as this will code for T7 lysozyme, which will suppress the T7 polymerase and can greatly reduce the level of background expression. If as stated before you have a protein that contains a large number of rare codons, then look for a h ost with the genes for the necessary tRNAs already present, which should allow your protein to express correctly. Sometimes simply changing hosts can have a dramatic effect on the amount of protein produced and the stability of the protein that is made, so if one host isnt giving you the results you need, then feel free to switch your host up. The third and final factor to consider when expressing a protein is growth conditions. When first starting out with the protein induction it is very important to run an expression time course, where you take a fresh colony from a streaked plate, and grow the culture to stationary phase. Next, dilute the overnight culture 1/100 and grow to mid log phase, then add the inducer and induce your protein for a number of hours, taking 1mL samples every hour or so. Once these samples are lysed, you can run an SDS-PAGE gel to determine your protein production levels. You might get great induction the first time, or you may have to tweak your conditions in order to get really good expression levels. Other factors that may need to be controlled for are the bacterial growth rate (determined by taking OD measurements during the induction process), and the temperature during induction. Some constructs will express perfectly fine at 37Â °C, while others need to be bumped down to 30Â °C to induce c orrectly. The concentration of the inducer too will have an effect, as many inducers (IPTG) can be toxic to the cells that they are inducing. Using freshly made inducer is good step to making sure you always have consistent results. Only through experimentation can you determine what will be best for your construct, and give you the most robust expression levels. Transformation efficiency: Transformation efficiency is a measure of the ability of cells to be transformed. Transformation efficiency is expressed as the number of transforms per microgram of pUC19. By using the following formula: Colonies on plate / ng of control DNA X 1000ng/Â µg = (transformation (T) / Â µg plasmid DNA) 100 ÃŽÂ ¼L equivalent to 0.01 ng DNA in the plate. Growth curve In general growth curve shows the S- shaped when plotted in log linear format as shown in figure 4, that separated into four phases: Lag phase; the initial period when no increase in cell number is seen. Log phase; when cells are growing at the maximumm rate. Stationary phase; growth decreases as a nutrient are depleted and waste products accumulate. Death phase; this is the result of prolonged starvation and toxicity. Conclusion The main goal for the experiment was to express the protein of interest (GFP). However, factors influencing transformation efficiency include technique errors, the temperature and length of the incubation period, the growth stage of the cells, and using the correct mass of plasmid DNA. Escherichia coli is one of the most important hosts in modern day recombinant protein production. Throughout academia and industry its uses are widespread and with sequence data available for some of the most common strains of the bacteria it has been a favourite organism for many metabolic engineering and metabolic modelling projects in the past (Berry, 1996; Koffas et al., 1999).

Shakespeares Othello - Desdemona the Wonderful Essay -- Othello essay

Othello: Desdemona the Wonderful  Ã‚        Ã‚  Ã‚   The innocent and charming personality of the wife of the general in William Shakespeare’s tragic drama Othello can hardly be rivaled – and yet she died the victim of a horrible murder. Let’s consider her case in this essay.    Louis B. Wright and Virginia A. LaMar in â€Å"The Engaging Qualities of Othello† comment on the virtue within the innocent wife of the Moor, and how pain came into her life:    Desdemona is warmhearted, tender, faithful, and much in love with her husband. No thought is further from her mind than the infidelity that Iago suggests to Othello. The suspense of the play increases as we watch Iago subtly poison Othello’s mind and witness Desdemona’s bewilderment, despair, and ultimate death, and this suspense is retained until the last lines when the spectator is left to imagine the tortures awaiting Iago, who is dragged off the stage to judgment.(129)    Just how innocent is the heroine? Robert Di Yanni in â€Å"Character Revealed Through Dialogue† examines the dialogue between Desdemona and Emilia, and finds that it reveals the former’s innocence:    In this dialogue we not only see and hear evidence of a radical difference of values, but we observe a striking difference of character. Desdemona’s innocence is underscored by her unwillingness to be unfaithful to her husband; her naivete, by her inability to believe in any woman’s infidelity. Emilia is willing to compromise her virtue and finds enough practical reasons to assure herself of its correctness. Her joking tone and bluntness also contrast with Desdemona’s solemnity and inability to name directly what she is referring to: adultery.(122)    Angela Pitt in â€Å"Women in Shakespeare’s Tra... ...   Di Yanni, Robert. â€Å"Character Revealed Through Dialogue.† Readings on The Tragedies. Ed. Clarice Swisher. San Diego: Greenhaven Press, 1996. Reprint from Literature. N. p.: Random House, 1986.    Pitt, Angela. â€Å"Women in Shakespeare’s Tragedies.† Readings on The Tragedies. Ed. Clarice Swisher. San Diego: Greenhaven Press, 1996. Reprint from Shakespeare’s Women. N.p.: n.p., 1981.    Shakespeare, William. Othello. In The Electric Shakespeare. Princeton University. 1996. http://www.eiu.edu/~multilit/studyabroad/othello/othello_all.html No line nos.    Wright, Louis B. and Virginia A. LaMar. â€Å"The Engaging Qualities of Othello.† Readings on The Tragedies. Ed. Clarice Swisher. San Diego: Greenhaven Press, 1996. Reprint from Introduction to The Tragedy of Othello, the Moor of Venice by William Shakespeare. N. p.: Simon and Schuster, Inc., 1957.   

The Yellow Wallpaper :: essays research papers

In The Yellow Wallpaper, a short story by Charlotte Gilman, there are many symbols within the text that one can construe a myriad of ways. One of the most prominent and perhaps the most important symbol is the titled yellow wallpaper. To the main character, Jane, the wallpaper is at first a nuisance, then an obsession, and finally salvation. The material of the paper itself represents Jane's everyday life. The illogical pattern that decorates it, reflects the absence of logic in her mind. The very color of the paper depicts the illness that yellows her sight and imprisons her within an unpredictable life. The wallpaper is at first a great annoyance to Jane; she claims that it is confusing and contradicting. Because her disease confuses her mind and contradicts her logic, the paper parallels her mental state at this point. Desperately attempting to unravel the mystery she imagines in the wallpaper, she becomes obsessed with deciphering its meaning. As her illness progresses, she begins to hallucinate and finally concludes that there is a woman trapped within that 'pointless pattern.'; Jane knows that she is the only one who can see the woman and, therefore, the woman's only chance of freedom. Slowly detaching from reality, Jane becomes the woman within the paper not only because of her obsession with it, but because of its parallel to her own life. In her final step toward insanity, she tears the paper off the walls to release the woman and herself. When her husband finds her, with the wallpaper and her sanity about her feet, she forcefully exclaims, 'I've got out at last...in spite of you and Jane. And I've pulled off most of the paper, so you can't put me back.

Lowering Testing Standards in Third World Countries Essay -- HIV AIDS

Lowering Testing Standards in Third World Countries ABSTRACT: Recently, Sidney Wolfe, director of Public Citizen’s Health Research Group (PCHRG), charged the National Institute of Health (NIH) and Center for Disease Control (CDC) with sponsoring fifteen immoral HIV studies in sub-Saharan Africa. The trials are being conducted to determine if certain alternate medical procedures or a short course of treatment with AZT, zidovudine or other drugs prevent some mother-child HIV transmissions. (1) Since the control group receives only placebos rather than AZT, Wolfe claims that the tests give suboptimal treatment that will result in more children contracting HIV and AIDS. (2) Public Citizen’s Health Research Group and others are calling for an immediate cessation of these important experiments. Public Citizen raises an important moral question. (3) Is it morally permissible to lower testing standards for the Third World? Unlike PCHRG, I contend that the answer to this question is yes, if the trials meet certain conditions. I e xplain both the First Best and Second Best Method (FBM and SBM, respectively) of testing new drugs and then compare the two. Next, I show the FBM’s impracticality in developing countries releases researchers from the moral obligation to use it. I then propose a new set of criteria — the Second Best Criteria (SBC) — that show if a test is moral or not. Finally, I argue that imposing a developed country’s moral standards for clinical trials in the Third World is immorally insensitive to the needs and conditions present in the latter area. Section 1 In clinical trials there are two different standard protocols for testing new drugs. The First Best Method tests the effects of a known drug (a benchmark), rathe... ...Biotechnology, vol. 11, pp. 135-143. Pederson, Daniel and Eric Larson, "Too Poor to Treat" Newsweek, 28 July 1997, p. 60. Rath, Richard, "Horton Hears the W.H.O." Dissonance, AIDS and Africa Resource page, 3 June 1996, http:/www.way.net/dissonance/nyrb_ltr.html, pp. 1-3. UNAIDS "HIV/AIDS: The Global Epidemic", Press Release, 28 November 1996, http:/www.us.unaids.org/highband/document/epidemic/situat96.html, pp. 1-15. —— "HIV/AIDS epidemiology in sub-Saharan Africa", 3 July 1997, http:/www.hiv.unaids.org/unaids/press/factafr.htm, pp. 1-3. —— "UNAIDS Announces New Clinical Trials for the Prevention of Mother-To-Child Transmission of HIV", Press Release, 9 July 1996, http:/www.hiv.unaids.org/unaids/press/mtctpren.html, pp. 1-2 No Author Listed: "AIDS in Africa", The Guide, 9 May 1996, http://www.guidemag.com/newsslant/aids.africa.html. pp.1-2

Cloud Computing

CLOUD COMPUTING PRASHANT G. PANDEY SANDEEP D. SINGH 3rd Author TIMSCDR TIMSCDR 3rd author's affiliation C-502, Silver Oak Appt. , Beverly park B-201, New Garden view, Tulinj Road, 1st line of address Mira Road (E), Thane-401107. Nalasopara (E), Thane- 401209 2nd line of address Mob. No. +91- 09322119824 Mob. No. +91- 09850394576 Telephone number, incl. country code prashant. [email  protected] com Sandy. [email  protected] com 3rd E-mail ABSTRACT As with any new trend in the IT world, enterprises must figure out the benefits and risks of cloud computing and the best way to use this technology. The buzz around cloud computing has reached a fever pitch. Some believe it is a disruptive trend representing the next stage in the evolution of the internet. Others believe it is hype, as it uses long established computing technologies. One thing is clear: The industry needs an objective, straightforward conversation about how this new computing paradigm will impact organizations, how it can be used with existing technologies, and the potential pitfalls of proprietary technologies that can lead to lock-in and limited choice. This document is intended to initiate a conversation that will bring together the emerging cloud computing community (both cloud users and cloud vendors) around a core set of principles. We believe that these core principles are rooted in the belief that cloud computing should be as open as all other IT technologies. This document does not intend to define a final taxonomy of cloud computing or to charter a new standards effort. Nor does it try to be an exhaustive thesis on cloud architecture and design. Rather, this document speaks to CIOs and other business leaders who intend to use cloud computing and to establish a set of core principles for cloud vendors. Cloud computing is still in its early stages, with much to learn and more experimentation to come. However, the time is right for the members of the emerging cloud computing community to come together around the notion of an open cloud. Multitenant : For an organization having many tenants renting some services. Authors & scientists of various publications: Hartig, Hinchcliffe, Greenberg, Robbins, Gupta & Swaminathan 1. INTRODUCTION The word Computing means any goal-oriented activity requiring, benefiting from, or creating computers. Thus, computing includes designing and building hardware and software systems for a wide range of purposes; processing, structuring, and managing various kinds of information; doing scientific studies using computers; making computer systems behave intelligently; creating and using communications and entertainment media; finding and gathering information relevant to any particular purpose, and so on. Computing is categorized according to the requirement of the individual or an organization. The topic I am going to discuss in this paper presentation would be â€Å"Cloud computing† dealing as Group and organization . Fig. 1 demonstrates a simple architecture about cloud computing. GENERAL TERMS â€Å"Cloud computing† is termed as the next big thing in the modern corporate world, the growing impact is will be seen on enterprise IT and business activities in many large organizations. This paper provides an insight to cloud computing, its impact and discusses the issues that business organizations face while implementing cloud computing, It also discusses various strategies that organizations need to adopt while migrating to cloud computing. Fig. 1 KEYWORDS Cloud computing is very simple and broad concept in which we are using internet which allows people access technology enabled services. It is named so as its representation is always done as a cloud as in a network diagram. In broader context, cloud computing can be said as a large network of computers used by large organizations to provide services to smaller ones and individuals. It is sometimes termed as Grid computing or Network computing. Cloud computing can be said as a Network of providing resource via network â€Å"on demand† and â€Å"at scale† in an multitenant environment. The resource provider network is called as â€Å"Cloud†. ? ? ? The wide band’s network fast development. The virtualization tech and market’s fast development. The hardware’s fast development, like CPU and network drives. 2. ISSUES NEEDED TO BE SOLVED I have come across certain issues which arise during the normal stages of computing which are needed to overcome, Let me take each issue one by one: 2. 1 Technical Issues The biggest challenge in cloud computing may be the fact that there is no standard or single architectural method. Common hurdles to be overcomed: Fig. Hartig States â€Å"What goes on in the cloud manages multiple infrastructures across multiple organizations consisting of frameworks providing mechanisms for self-healing, selfMonitoring and automatic reconfiguration†. The cloud is virtualization of resources so it manages itself. Still there are people required to take care of operating systems, hardware and networking in a proper or der, but from the user’s or application developer’s perspective, only the cloud is referenced. We can say cloud computing is the Third revolution of IT industry, following the personal computer revolution. Cloud computing matters to us as cloud computing and web based are the future of computing in which all of us will interact. Let me give you an examples that in our day to day life, we come across a number of vendors providing cloud computing services such as Google, Yahoo, MSN, etc. among web based office applications and online photo and document sharing include flickr and Zoho. By cloud computing, we have the ability to scale to meet changing user demands quickly, usually within minutes. Cloud computing is : ? Environment friendly ? Task oriented ? Requires no Maintenance It gives user the benefit of separating application code from physical resources and to use external assets to handle peak loads. 2. 1. 1 Security Security is the most important thing for an organization or and company. If a company has a very big portal then we cannot say that the company is really very big but if the security of the company should be well assured that it is safe . Hence, I can say that Security is the most important hurdle for an organization to keep its data & policies undercover so that only the recommended people can have an access to it. With their business information and critical IT resources outside the firewall, customers worry about their vulnerability to attack. 2. 1. 2 Technical Hardware & Software Expertise Users need equipment and resources to customize cloud computing services more relevant and more tailored to the needs of their businesses. Proper man-power is needed to develop the applications to suit a business’s needs. The availability of physical hardware and software components need to be ensured for realizing the benefits of cloud computing. According to the writings of Hinchcliffe(2009, p. 61), wider technical fluency and expertise in the selected cloud computing platforms, which tend to emphasize technologies such as Open Source or newer web-style programming languages and application models will have to be achieved. 2. 2 Non-Technical Issues needed to overcome Apart from the technical issues, there are several non-technical issues which require equal attention and need to be resolved. Some of the significant non-technical hurdles to the adoptions of cloud computing services by large enterprises are financial, operational and organizational issues. . 1 Reasons to accept Cloud Computing. ? ? ? The fast increase of corporate IT infrastructure requirement. Economy crisis forcing company to cut cost. The fast change and time-to-market requirement of Internet applications. 2. 2. 1 Financial Issues Different people have different opinions on cloud computing, One such report says , Cloud computing can cost twice as much as inhouse data centers. This poses a problem for large enterprises, but actually works to the advantage of small and midsize companies and businesses. Further reports say that Cloud offerings are most attractive for small and medium-sized enterprises†¦ and most customers of clouds are small businesses. The reason behind this is that smaller companies don’t have the option of developing themselves into giant data centers. Greenberg(2009) notes, â€Å"Few if any major corporations are looking to replace their data centers with cloud†¦ the ‘server-less company’ are one that’s only feasible for startups and SMBs. † Cost variability is the important aspect of cloud computing. If cost ,transparency, scalability and cost variability is to be considered then a new challenge and opportunity arises for organizations. security through the public cloud. The availability of a robust network and information security is also a challenge. 2. 2. 3. 3 Cost Cost of process change is another hurdle in the transformation. Conventional IT organizations will have to engage with internal customers as well as IT service providers at a different plane. Most importantly, the culture and mindset and mindset will have to change. 3. PLANNING TOWARDS TECHNOLOGY THE NEW 2. 2. 2 Operational & Organizational Issues Organizations need to define standards and workflow for authorizations. A strategy for the consumption and management of cloud services, including how the organization will deal with semantic management, security and transactions need to be created. One should evaluate cloud providers using similar validation patterns as one does with new and existing data center resources. According to Gupta (2008), before deciding to switch over to cloud computing, one should fully understand the concept and implications of cloud computing as to whether maintaining an IT investment in-house or buying it as a service. The organization has to look at the overall return on investment inhouse or buying it as a service. The organization has to look at the overall return on investments as they cannot simply rip off and replace an existing infrastructure. The managers have to look at the short-term costs as well as the long term gains. Finally, a proof of concept should be created which can do a few things including getting an organization through the initial learning process and providing proof points as to the feasibility of leveraging cloud computing resources. Cloud computing is inevitable and it is a force that organizations and businesses need to quickly come in terms with. As the economic and social motivation for cloud computing is high, businesses which are heavily computer resource dependent need to take cautionary measures and the right decisions at the right time to avoid ending up with unreproductive solutions while migrating to new technologies. According to Robbins(2009), an organization should always make sure that they know what they are paying for and should pay careful attention to the following issues: ? Service levels ? Privacy matters ? Compliances ? Data ownership ? Data mobility. A number of cloud computing vendors may be hesitant to commit to the consistency of performance regarding an application or transaction. One has to understand the service levels they expect regarding data protection and speed of data recovery. 4. SWITCHING TO NEW TECHNOLOGY 2. 2. 3 Internal Issues While switching to newer technologies, an organization could face many internal issues. Some of them are explained as follows: Switching to newer technology such as cloud computing would be best when the processes, applications, and data are largely independent. When the points of integration in a business are well defined, embracing cloud services is effective. In an organization where a lower level of security will work just fine and the core internal enterprise architecture is healthy, conditions are favorable for the organization to switch to newer technologies. A business which requires Web as the desired platform to serve its customers 2. 2. 3. 1 Distributed business levels The distributed business and the level of consistently reliable computer networks in an organization can pose a challenge towards switching from traditional infrastructure to cloud computing. The case for an organization to go in for cloud computing is similar to a decision to own or rent a house. An organization which has spent a good amount of cash on its own storage and security systems will have time taking the decision to migrate to dedicated environment. 2. 2. 3. 2 Complexity of applications The complexity of the applications and the technology infrastructure is dependent on how the organization has adopted IT. If this has evolved from the deployment of technologies over a period of time, then the complexity level will certainly be high and in such a case, transformation to cloud computing would be difficult. Not everything comes under cloud computing as each organization has its own specific requirements suited to their needs whether on functionalities, performance, or maybe even computing in their organizations. Unless they seriously consider making cloud a part of their strategy. References: [1]. Buyya, Rajkumar; Chee Shin Yeo, Srikumar Venugopal, Sudershan Malpani (PDF). Market-Oriented Cloud Computing: Vision, Hype, and Reality for Delivering IT Services as Computing Utilities. Department of Computer Science and Software Engineering, The University of Melbourne, Australia. p. 9. [2]. www. gridbus. org/~raj/papers/hpcc2008_keynote_cloudcom p2uting. pdf. Retrieved 2008-07-31. [3] . M. D. Dikaiakos, D. Katsaros, G. Pallis, A. Vakali, P. Mehra: Guest Editors Introduction: â€Å"Cloud Computing, IEEE Internet Computing†, 12(5), Sep. 2009. [4] San Murugesan (Editor), â€Å"Cloud Computing: IT's Day in the Sun? â€Å", Cutter Consortium, 2009. [5] Luis M. Vaquero et al. , A Break i n the Clouds: Toward a Cloud Definition, ACM SIGCOMM Computer Communication Review, Volume 39, Issue 1 (January 2009), Pages 50–55, ISSN:0146-4833 [6] [7] www. ikipedia. org/cloudcomputing/ www. scribd. com/cloud/cloudppt. ppt/ Fig. 3 and wants to cut cost while benefiting from the new applications, the business can achieve the best competitive advantage in the market. According to Swaminathan (2009. p. 14), to compete effectively in today’s world, executives need every edge they can get, from low cost to speed and employee productivity. By tapping into the right cloud capabilities, companies can quickly enter new markets and launch new products or services in existing markets. When demand grows, they can quickly scale up, and when opportunities dry up, they can just as quickly scale down with minimum waste of time and capital. By using cloud-based solutions such as crowd-sourcing, companies can open up innovation to more employees, customers and their partners. 5. CONCLUSION Cloud computing is a fascinating realm, that makes it easier to deploy software and increase productiovity. However, there are some technical and non-technical realities that make security somewhat difficult to deliver in cloud. The cloud presents a number of new challenges in data security, privacy control, compliance, application integration and service quality. It can be expected that over the few years, these problems will be addressed. According to Swaminathan(2009, p. 12-15) and Daugherty(2009, p. 12-15), to be successful, companies should take small incremental steps towards this new environment so they can reap benefits for applicable business situations and learn to deal with the associated risks. In general, Cloud computing will act as an accelerator for enterprises, enabling them to innovate and compete more effectively. Under the current economic conditions, executives need to rethink their strategies dealing with cost-effective solutions. They need to use the cloud services for the right jobs they require. Today’s infrastructure clouds such as Amazon EC2 offer a relatively inexpensive and flexible alternative to buying in-house hardware. They are also beneficial for computation-intensive hobs, such as data cleansing, data mining, risk modeling, optimization and simulation. Businesses and enterprises should now take steps to experiment, learn and reap some immediate business benefits by implementing cloud Cloud Computing Each group Is expected to work on a project and by end-term, submit a hard copy of the project and also, In summary, present the findings. The project should reflect your learning's from the BIT course. Hence, the topic selection has to be such that your project should address one of the following two Study In depth any ONE area of IT Investments In an organization. The study has to highlight the business objectives addressed by the system approach to implementation analysis of success or failure of the implementation.What you would have done differently – give alternative solutions and recommendations Finally, what Business related benefits were derived from the IT investments It will be Important to analyze in light of Management, Organization and Technology aspects of Implementation. Study of ONE technology for Its applicability, usefulness and Its Implications for organizations across an Industry. Study the business objectives for which the Information system was Implement ed In different organizations.You study should cover the following analysis: Is purpose and need for hat particular technology Information system the same across all companies studied? Were the business goals the same for the technology implementation? Did all companies face the same risks, problems and challenges in implementing the technology within the company? Did you observe any common trend or practice emerging across companies of the same industry? Finally, what Business related benefits were derived from the IT investments Suggested list of technologies and industries that can be studied: Technologies IndustriesCloud computing, AAAS, alas, Fertilization Retail Enterprise applications Like Banking, Financial services e-Business & e-commerce Insurance Social media Manufacturing, Automobile Data-warehouse and Bal Telecoms You can select your own technology or combination of technology and industry. Submission of Synopsis Each group Is expected to write a synopsis ( not more tha n 2 pages) of the project the group intends to do. Synopsis will cover the Topic of the project Approach the group intends to follow Broad sections of the reportDivision of effort – list the responsibilities allocated to each member of the group Project Report Project report will be submitted in soft copy. The report should tallest be of 12 pages and not exceed more than 25-30 pages. Your report should reflect your group's analysis and effort. Any report which I find has been directly downloaded from one source and reproduced will be completely rejected and no marks will be given to that group. Important dates 1 . Submission of synopsis: Gag 31st 2. Project submission: End of 18th session. The date will be confirmed by Seep 4th Cloud Computing These combine together and deliver services such as the delivery of software, Infrastructure and storage. There are different types of cloud computing. There are public clouds, private clouds and hybrid clouds. Public clouds are virtual data center. A service provider such as Dropped makes resources available over the Internet. Private cloud are usually inside an organization's firewall but it also could a private space dedicated to the organization provided by a service provider. Hybrid cloud combines both aspects of public and private cloud.What are the advantages of cloud computing? Cloud computing is very cost efficient as it is very easy to maintain and use. Cloud computing Is a lot cheaper than traditional software. As cloud computing is very high productive, It saves a lot of money and time. Besides cost efficiency, one of the advantages of cloud computing is that most cloud service providers are usually competent to recover information. It is much easier to backup and recover information than on physical storage device. Another advantage of cloud computing would be Its automatic software Integration function.Automatic software Integration means a person does not take hard effort to customize and Integrate applications. Cloud computing allows the person to customize easily. Not only it's very easy to customize, cloud computing is also very easy to access information. A person can easily access the cloud from every single corner In the world with Internet access anytime. What are the disadvantages of cloud computing? Cloud computing offers a lot of positive aspects. But, It also has Its downside. From time to time, cloud computing system face some serious technical issues.Sometimes it is dysfunctional despite it is constantly maintained. Security issue is also one of the biggest concern in cloud computing. If an organization is using a cloud computing service provider, confidential personal information or other sensitive Information could be exposed to th e third party. Hence, it is important to make sure that a cloud service provider you're going to choose is secure. Cloud computing is very easily exposed by hackers or any kinds of threat because anything on the Internet is vulnerable to attacks.Cloud computing has made it very easy to access Information but one of the biggest flaws Is that It doesn't provide with a lot of space. Most of the cloud service providers only provide less than BIBB of space to its user. A business 1 OFF organization chose a service provider, the organization depends a lot on the service provider. It would be very troublesome to switch from a service provider to another. It would take a lot of time to transfer all the files. How could a Canola Computer Store customer use Dropped? Dropped can store photos, documents, videos and files for its users.Dropped has applications for several platforms such as computer, mobile phones and tablets. Dropped is also accessible on the Internet. Dropped also allows its us ers to share their folders with whomever they desire. Besides sharing a folder, Dropped users can also send links of specific files, photos and folders. Dropped is a free service and you can sign up at www. Dropped. Com. Part B: Disposal of Data and Computer Components What are the two methods of secure data disposal? Sims Recycling Solutions offers two kinds of secure data disposal.They offer data wiping and data shredding method. How does each method disposes the data? Sims Recycling Solutions uses the data wiping method by using the industry leading data cleaner software called Balance to wipe the data in the hard drive. Data herding method is also used by Sims Recycling Solutions. Data is disposed by physically shredded by large shredding machines. Why dispose data using data wiping method? Data wiping is an excellent method for reusing purpose. Some organization uses the data wiping method to wipe all the data then remarked it and sell.By using data wiping method, an organizati on can rescue components of an equipment for maintenance and sale purposes. Data wiping also allows an organization to refurbish an equipment and donate for charities as it wipes all the data inside. Why dispose data using data shredding method? Data shredding is perfect if reusing an equipment is not an option hence requires data shredding method to physically destroy the equipment. It is also a good choice for Solid State Memory and non-magnetic storage devices such as DVD's and compact disc (CD) because these kinds of storage devices need data shredding method to destroy them securely.How Sims Recycling Solutions deals responsibly, ethically and in an environmental friendly manner with electronic waste? Electronic waste such as computer monitor and television contain substances that are harmful to the environment if they are not disposed carefully. Sims Recycling Solutions offers to collect electronic waste such as computer monitor and television to local Sims Recycling Solution disposal centre. We use Sims Recycling Solutions because the company recycles hazardous electronic waste in an environmental friendly way.The company also recycle as much as they can. The company is able to recycle the glass in screens. Photocopiers, toner cartridges and printers can also be recycled. Sims Recycling Solutions has the technology to break toner cartridges for energy recovery and recycle them into raw material in an environmental friendly way. Recycling effective and environmental friendly. Sims Recycling Solutions ensures 100% data disposal in printers and photocopiers as they record everything printed or photocopied in their memory. Where can I dispose IT and computer?

Case Study: Chrysler-Fiat Partnership Essay

When America’s economical crisis reached its apex, domestic car manufacturers were at the forefront of struggling industries, and Chrysler was one of the hardest hit (Car and Driver, 2008). In 2008 the automotive giant, along with fellow industry stalwart General Motors, received a $17.4-billion reprieve from the American Government to keep from closing its doors altogether (Car and Driver). Chrysler did lose a lot of respectability, and was ordered to cease and desist with any new product development until the company proved it could be a viable business (Gluckman & Kurczewski, 2009). However, the loan from the government proved to still not be enough to get Chrysler back on its feet, and in 2009 the company filed for Chapter 11 bankruptcy (Groth, 2011). Fiat faced its own organizational struggles in 2003-2004 before new CEO Sergio Marchionne led the Italian automotive manufacturer back to respectability (Gluckman & Kurczewski). Still, after watching European car sales fall to a 17 year low and needing a boost to his company’s revenue, Marchionne saw the Chrysler situation as a way to get into the American market (The Economist, 2013). Objectives Sought by Each Partner: Chrysler’s objectives in the partnership with Fiat were pretty simple: it needed a financial boost to maintain its place in the industry and new technology if it wanted to grow and advance (Marrs, 2009). After egregiously unsuccessful partnerships with Daimler-Benz and Cerberus Management Group and a multi-billion dollar loan from the American Government ended with Chrysler filing for bankruptcy, the company was in desperate need of a method to regain viability (Marrs; Krisher & Strumpf, 2009; Gluckman & Kurczewski, 2009). Although Chrysler received no money in the deal, it will emerge as a new, leaner group minus billions in debt, 789 underperforming dealerships, and burdensome labor costs, not to mention gaining Fiat’s  technology to build new environmentally friendly, fuel efficient, high-quality vehicles (Krisher & Strumpf). Fiat’s objective in the partnership was to provide a financial boost to its own company without accumulating additional debt (Ebhardt, 2013). Fiat, Italy’s largest auto manufacturer, would like to expand its market to become a global competitor. Fiat CEO Sergio Marchionne believes that to compete with General Motors, Volkswagen, and Toyota, the merged Fiat-Chrysler will need to produce 5.5-6 million cars a year, compared to its current output of 4.1 million (The Economist, 2013). Basis of Dialogue Leading to the Partnership: The basis of a dialogue leading to a potential partnership was the concept of a mutually beneficial situation for all parties involved (Cox, 2013). Fiat has the capital, new technologies to develop high-efficiency cars, and reverence from Ferrari and Maserati fans that will allow Chrysler to regain its place among top domestic auto manufacturers in the United States (Groth, 2013). Fiat will share with Chrysler its platforms and powertrain technology, including engines, transmissions, and fuel-saving technology (Gluckman & Kurczewski, 2009). Through Fiat, Chrysler will also get better distribution of its products in Europe, India, Brazil and China (Gluckman & Kurczewski). Chrysler is the 3rd-largest U.S. auto company and is a trusted brand with the international appeal, customer base, and facilities that will allow Fiat to become a serious competitor in the global automotive manufacturing market (Groth). Chrysler was also in no position to be patient for an extended period of time. While its factories sat idled during the bankruptcy process, the automaker reportedly lost 100 million per day (Krisher & Strumpf, 2009). Steps Taken by Each Company: The partnership between Fiat and Chrysler, which is still an ongoing process, is being approached in phases. Initially Chrysler agreed to give Fiat a 35% holding in return for an influx of new engines and platforms, research and development, and help retooling its plants (Marrs, 2009). This approach allowed both organizations to ease into the partnership, without either side immediately taking on too much debt or risk (Cox, 2013). Analysts were not  able to exactly predict the partnership between Fiat and Chrysler. In fact, Chrysler was in talks with General Motors before both companies began to experience serious financial hardships (Gluckman & Kurczewski, 2009). Looking to avoid the management mistakes that doomed Chrysler’s partnerships with Daimler and Cerberus, Fiat CEO Sergio Marchionne has made it clear that Fiat/Chrysler will run as one company (Trujillo, 2013). As Mr. Marchionne announced at a media briefing, â€Å"This management team spends their time traveling and making decisions, but this thing runs as one house. There is no question about who runs what; I run one company† (Vlasic, 2013, pp. 4).